FIG. 7.

Em492 antigen does not exhibit a cytotoxic effect on splenocyte populations. (A) In vitro-cultured splenocytes were stimulated either with ConA alone or with ConA in the presence of Em492 antigen. Following trypan blue staining of cells, the numbers of dead and live splenocytes were determined microscopically in a Neubauer chamber by inspection of all nine quadrants of the chamber and determination of the ratio between dead and live cells. The data shown correspond to one out of three experiments, with all three yielding largely identical results. (B) TEM of ConA-stimulated splenocytes. Note the macrophage in the process of phagocytosing a dead cell. (C) TEM of splenocytes incubated in the presence of ConA plus Em492 antigen. Bars = 28 μm.