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Cell wall distribution of melanin in F. pedrosoi. Sclerotic cells from patient lesions (A and B) or from in vitro cultivation (C and D), conidia (E and F), mycelia (G and H), or melanin ghosts (I and J) were incubated with antibodies against melanin followed by FITC-labeled anti-human antibodies and were analyzed by fluorescence microscopy. Left panels show fungal cells under differential interference contrast, while right panels show the same images under fluorescence. Bar, 10 μm.
