FIG. 7.

Influence of soluble melanin in the oxidative burst of human neutrophils during incubation with F. pedrosoi conidia (A) or sclerotic bodies (B). The degrees of oxidative burst were measured by the emission of fluorescence after the oxidation of DHR to rhodamine. Bars represent the intensity of rhodamine fluorescence after interaction of F. pedrosoi with neutrophils in the absence (black bars) or presence (white bars) of soluble melanin. In the presence of soluble melanin, oxidative burst was significantly enhanced after interaction of neutrophils with conidia (P < 0.005) or sclerotic forms (P < 0.01). Results are expressed as means of three independent experiments ± standard deviations.