Fig. 5.

Expression of a temperature-sensitive potassium channel in human corneal endothelial cells (HCEC) immortalized with human telomerase reverse transcriptase (hTERT) and CDK4. HCEC-CDK4/TERT cells (Zante at beyond PD 100) were used in these experiments. A single cell was voltage clamped at −5 mV and voltage gated macroscopic currents recorded at (a) 22 °C and (b) 34 °C by stepping the potential between −65 and 75 mV. An outward rectifying potassium current is activated in these cells by raising the temperature as illustrated by the current voltage plot in (c) (n = 4).