FIG. 1.

BCG dose-dependent immune activation. Three doses of BCG were administered s.c. (A) or i.n. (B) to BALB/c mice. After 3 weeks, splenocytes were pooled from two mice per treatment and were stimulated with mycobacterial antigens (cBCG) or unrelated antigen (keyhole limpet hemocyanin [KLH]). IFN-γ in the culture supernatant was measured. Results are expressed as means ± standard errors of the means from triplicate determinations per condition.