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Kinetics of immune responses triggered by s.c. or i.n. BCG vaccination. A dose of 5 × 105 CFU of BCG was administrated s.c. or i.n. to BALB/c mice. At weeks 3, 6, and 9, splenocytes (A) and thoracic lymph node (LN) cells (B) were isolated and pooled from two or three mice per group and cultured with or without mycobacterial antigen cBCG. IFN-γ in the culture supernatant was measured. Results are presented as means ± standard errors of the means from triplicate determinations.
