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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Harmful Algae. 2012 Sep;19:85–91. doi: 10.1016/j.hal.2012.06.003

Table 1.

Comparison between radioligand and fluorescent ligand brevetoxin receptor assays.

Compound Ki: Fluorescent-ligand Assay Ki: Radioligand Assay
PbTx-1 0.68 +/− 0.12 0.66 +/− 0.10
PbTx-2 1.03 +/− 0.30 1.09 +/− 0.06
PbTx-3 1.31 +/− 0.30 1.55 +/− 0.11
PbTx-9 3.63 +/− 0.40 3.80 +/− 0.47

A series of experiments were performed to compare the Ki values obtained for PbTx-1, PbTx-2, PbTx-3, and PbTx-9 between the radioligand and fluorescent ligand PbTx-2 receptor assays. Experiments were performed on the same day, using the same batch of synaptosomes, and assaying the same dilutions of compound (1pm to 1µM). Each value represents the mean Ki +/− SEM from 6 independent tandem experiments. There was no significant difference in Ki values between assays for any competitor shown (p < 0.05).