Figure 3.

Functional complementation tests of CYP97/HYD enzyme combinations in cells accumulating α- and β-carotene. E. coli cells accumulating both α-carotene and β-carotene were transformed with the combinations of test plasmids HYD4 + CYP97C2 and CYP97A4 + CYP97C2. Extracted pigments were separated by reverse-phase HPLC, with spectra extracted at 450 nm. Control, Empty pCOLADuet; L, lutein; Z, zeaxanthin; Zei, zeinoxanthin; βcr, β-cryptoxanthin; cβ, 13-cis-β-carotene; αc; α-carotene; βc, β-carotene.