Figure 3.

Inactivation of ETHE1 disrupts seed development. A, Mature siliques of wild-type, ETHE1/ethe1, and ethe1 complementation plants observed by light microscopy. Aborted seeds are indicated by arrows. Size bar = 1.5 mm. B, ETHE1/ethe1 developing siliques observed by light microscopy. Ethe1^−/− seeds are indicated with an asterisk. C to E, SEM of developing seeds in ETHE1/ethe1 siliques. C and D, The outer integument in post-globular-staged wild-type and ethe1 seeds. Higher magnification image of the outer integument of an earlier staged wild-type seed is shown in section E. F and G, Confocal microscopy of Wt (F) and ethe1 (G) seeds from the same silique at approximately octant stage. Size bar = 10 μm. [See online article for color version of this figure.]