Figure 5.

Immunolocalization of ETHE1 in wild-type buds. Sections of wild-type buds (A and B), anthers (C and D), and seeds (E–O) were prepared and treated with preimmune serum (A, C, E, H, J–l) or ETHE1 antibody (B, D, F, G, I, M–O). ETHE1 cross-reactive material is purple, nonspecific signals from the detection system are brown. ETHE1 is present in the tapetal cells of the anther (B and D), zygote (F), and PEN nuclei (G). ETHE1 is also present in globular-staged seeds (I), in particular, in the globular embryo (M), the PEN nuclei (N), and the chalazal cyst (O). The embryo and chalazal region are labeled with e and chz, respectively. Size bar = 50 μm.