Figure 2.

Overexpression of GNC induces chloroplast biogenesis in the cotyledon epidermis, hypocotyls, and roots. First row, 7-d-old seedlings of Col-0, gnc cga1, and two independent GNC overexpression lines (OX-1 and OX-2) grown under constant white light. Bars = 0.5 mm. Arrows show the hypocotyl-root junction. Second row, detection of chlorophyll autofluorescence in the cotyledon adaxial epidermis of 14-d-old seedlings grown under constant white light. Bars = 20 µm. Third and fourth rows, detection of chlorophyll autofluorescence in the hypocotyls of 7-d-old seedlings (third row) and in the roots of 10-d-old seedlings (fourth row) grown under constant white light. Bars = 0.1 mm. Chlorophyll autofluorescence and cell wall staining by propidium iodide were false colored in green and magenta, respectively.