Figure 7.

Inducible expression of GNC simulates postembryonic chloroplast production in seedlings. A, GNC expression following 10 μm DEX induction for 4 h as determined by qRT-PCR. Expression was determined for Col-0, the gnc cga1 mutant, and a gnc cga1 line carrying DEX-inducible GNC (DEX-L7). Error bars indicate sd. B, Effect of GNC induction on chloroplast biogenesis. Seven-day-old seedlings of the gnc cga1 mutant and DEX-L7 were grown in the presence or absence of 1 µm DEX under constant white light. Bar = 0.5 mm. Detection of chlorophyll autofluorescence in the cotyledon abaxial epidermis of 7-d-old seedlings is also shown. Chlorophyll autofluorescence and cell wall staining by propidium iodide were false colored in green and magenta, respectively. Bar = 20 µm.