Figure 7.

LBD20 is a repressor of a subset of JA-regulated defense genes. A to C, LBD20, Thi2.1, and VSP2 expression was examined in shoot tissue of wild-type (WT) and LBD20-OX lines 6 h post mock or MeJA treatment. LBD20-OX lines 1 and 2 are in the WT background, while lines 3 and 4 are in the lbd20-2 mutant background. Gene expression levels are relative to the internal control β-actin genes. The average of three biological replicates consisting of pools of 30 plants is shown with se. Asterisks indicate values that are significantly different (**P < 0.01, *P < 0.05 Student’s t test) from WT under the same treatment. No significant differences in expression levels among mock-treated samples were observed for Thi2.1 or VSP2. D, MeJA-induced expression of defense marker genes in LBD20-OX lines was plotted against their internal LBD20 expression. Displayed are trend lines.