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. 2012 Jul 12;160(1):450–463. doi: 10.1104/pp.112.198200

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 5. — Inhibition of Pro hydroxylation of MUC1 in BY-2 cells. A, MALDI-TOF-MS analysis of endoproteinase Asp-released MUC1 tandem repeats (DTRPAPGSTAPPAHGVTSAP; indicated by arrows) from Tn-GF(MUC1)P expressed in BY-2 cells coexpressing CytoEpi-T2. The analysis revealed the presence of 3 mol of attached GalNAc (i.e. complete GalNAc-T2-mediated GalNAc occupancy of sites), with varying Pro hydroxylations of up to 4 mol per tandem repeat. Hydroxylated MUC1 peptides were further modified by the attachment of mainly three, and less frequently of six, Ara residues. B, MALDI-TOF-MS analysis of MUC1 tandem repeats (indicated by arrows) of GF(MUC1)P coexpressed with CytoEpi-T2 in BY-2 cells grown in the presence of a P4H inhibitor. Growth medium was exchanged at day 7 with fresh medium including 200 µm 2,2-DP, followed by incubation in similar growth conditions. [See online article for color version of this figure.]