Experimental validation of the mathematical model. A, Model predictions of the root length when only the BRI1 receptor concentration and ligand concentrations are altered. At 62 nm, the model predicts the Col-0 wild-type root length, 31 nm represents the heterozygous bri1-116 line, and 2 and 0 nm represent the bri1-301 and the homozygous bri1-116 lines, respectively. B, Experimental verification of the model predictions using Col-0, bri1-116 heterozygotes, bri1-116 homozygotes, and bri1-301 homozygotes. Error bars represent se for 30 roots per data point measured in three independent replicates.