Figure 3.

The BRI1-GFP lines are more sensitive to BL. A, Model prediction for the behavior of a BRI1-GFP line. B, Root length assay of BRI1-GFP lines 1 and 2 containing two and three times more BRI1, respectively. The effect of the additional BRI1 copy is more severe than what the model predicts. All points in the graph are shown ± se for 30 roots per data point measured in three independent replicas. C, The model is able to fit the results of the BRI1-GFP lines. All parameters were fit to the BRI1-GFP lines and Col-0 wild-type lines separately. The BRI1 concentrations used for modeling are 62 nm for Col-0, 120 nm for BRI1-GFP line 1, and 200 nm for BRI1-GFP line 2. D, The behavior of BRI1-GFP lines can only be modeled when k₁, k₂, and k₃ are altered when compared with the Col-0 lines. The following values for k₁, k₂, and k₃ were used for modeling: 2 nm for Col-0, 0.5 nm for BRI1-GFP line 1, 0.2 nm for BRI1-GFP line 2, and 15 nm for bri1-301. The maximum root length and the minimum root length were 12 and 0.35 cm, respectively.