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. 2012 May 5;71(11):1872–1880. doi: 10.1136/annrheumdis-2011-201180

Figure 3.

Figure 3

Dexamethasone (Dex) attenuates K/BxN arthritis in AnxA1+/+ but not AnxA1−/− mice. AnxA1+/+ and AnxA1−/− mice were given an intraperitoneal injection of K/BxN serum (50 μl at day 0 and day 2) and then received vehicle or Dex (10 µg intraperitoneally daily). (A) Arthritic scores as monitored over a 10-day time course. *p<0.05 versus appropriate vehicle control (two-way analysis of variance). (B) Right ankles of mice from each experimental group were taken at day 10 and joints processed for staining by H&E and safraninO. Representative images are shown. Scale bars, 50 μm. CE, cartilage erosion; PF, pannus formation; S, synovitis. (C) Histomorphometric analyses of joint sections. *p<0.05 versus appropriate vehicle control (Student t test). (D) Quantitative real-time PCR was performed on cDNA from left ankle joints; pro-inflammatory genes were analysed with all Ct values normalised to endogenous Gapdh (four mice per group). RQ values were calculated using 2−(ΔΔCt) and data shown here as mean % gene inhibition by Dex relative to vehicle control groups±SE. Naive joints were set as the calibrator samples. *p<0.05 versus respective vehicle control (Student t test).