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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 13;189(4):1639–1647. doi: 10.4049/jimmunol.1200528

Figure 1.

Figure 1

Null mutation of IEX-1 enhances Th17 differentiation. WT and IEX-1-knockout (KO) CD4+ T cells were incubated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 for 4 days (Th0) or along with 1 ng/ml TGF-β and 20 ng/ml IL-6 (Th17) followed by intracellular staining and FACS analysis for IFN-γ versus IL-17 expression (A). The CD4+ T cells were also differentiated under Th17-polarizing conditions as A except that an increasing concentration of TGF-β (B) or IL-6 (C) was included in the culture. D. The CD4+ T cells were differentiated under Th17 conditions in the presence or absence of 50 ng/ml IL-23 or 10 µg/ml anti-IL-2 antibody as A. Representative flow cytometric profiles are shown on the left and mean percentages ± SD of Th17 cells are summarized on the right in each panel. *, ** and ***, p<0.05, 0.01 or 0.001, respectively, in presence or absence of IEX-1 (n = 6 for all).