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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 13;189(4):1639–1647. doi: 10.4049/jimmunol.1200528

Figure 3.

Figure 3

ROS increase IL17 transcription in the presence of RORγt. HEK293 cells were transfected with a RORγt expression vector (RORγt) or empty vector (Vector) along with a minimal CNS2-Il17 promoter-luciferase reporter (A). The cells transfected with the RORγt plasmid and Il17 promoter-CNS2 luciferase reporter were treated with 0, 1 or 5 mU/ml of glucose oxidase (GO) (B) or 0, 1, 5, or 10 mM of NAC (C). The resultant cells were lysed in 24 hr and luciferase activities were assayed in which renilla luciferase activity was used for the normalization of transfection efficiency and background luciferase activities in control cells transfected with empty vector alone were set to 1. The data are expressed as mean fold increases ± SD (A) or mean percentages ± SD (B and C) of luciferase activity values relative to control cells from three separated experiments each performed in duplicate. * and ***, p<0.05 or 0.001, respectively, compared to the values in control cells.