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. 2012 Sep 12;7(9):e45221. doi: 10.1371/journal.pone.0045221

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© 2012 Ben-lulu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Relative expression of p-ERK, β-catenin, IL-1B and Bax proteins in jejunum and ileum as determined by Western blot analysis of rats following MTX induced mucositis. Values are mean ± SEM. CONTR-control, MTX-methotrexate, TGF-β -transforming growth factor beta. *P<0.05 MTX, MTX-TGF-β and CONTR-TGF-β versus CONTR rats, ^†P<0.05 MTX- TGF-β versus MTX rats. ERK2 was detected using anti-ERK2 antibody to verify equal protein loading.