Table 1. Oligonucleotides used for the multiplex PCR reactions.

	VNTRa	Oligonucleotide	TM °C	Repeatsize (bp)	Size of flanking regions (bp)b	Allele range (bp)c
Multiplexe 1	Abaum_3406_L_D4	CACTATATTGAAGTGCTTTTA	48	30	231	342	669
	Abaum_3406_R	GTGGTTTTTCTATTGGTACATTAC	58
	Abaum_826_L_D3	TGACTACTGAAACAGTTTTTG	56	9	361	415	649
	Abaum_826b-R	ACTTGGTTTGAGCTATAGAA	54
	Abaum_17_L_D3	GTGAGGGTAGAGTATTTGCTC	62	9	80	85	346
	Abaum_17_R	GAGTTAGGGAGTCTTTTATATGG	64
	Abaum_845b_L_D2	CCAAATTGCTCCAATATCTGAACT	66	7	115	129	346
	Abaum_845b_R	GCAAATTTATACAGCTCAAGAAAATGG	72
	Abaum_2396_L_D4	CAAGTCCAATCAACTCATGATG	62	6	105	147	315
	Abaum_2396_R	CTCCTGTAAGTGCTGTTCAGCC	68
	Abaum_3468_Lb_D2	AAGACCACTTTGCAACTAAA	58	6	308	350	429
	Abaum_3468_Rb	TTTGCTGATGGTTGTGGCTA	58
Multiplexe 2	Abaum _3530_L_D2	TGCAACCGGTATTCTAGGAAC	62	60	121	162	642
	Abaum _3530_R	CCTTGAACAACATCGATTACTGGA	66
	Abaum_3002_L_D3	GACTGAAGCAAGACTAAAACGT	62	57	121	428	596
	Abaum_3002_R	TCTGGGCAGCTTCTTCTTGAGC	68
	Abaum_2240b_L_D4	AAGGTTTCCGTGTTGAACGTGATT	68	99	209	204	538
	Abaum_2240b_R	CAGGTGTCAAATCGTAAACAAA	60
	Abaum_1988_L_D3	GGCAAGGCATGCTCAAGGGCC	70	26	77	136	447
	Abaum_1988_R	CAGTAGACTGCTGGTTAATGAG	64

^aThe forward primer is labeled with one of three dyes D2, D3 and D4. The allele range for each reaction is indicated showing that amplicons with the same dye cannot overlap.

^bValues referred to the genome of strain ATCC 17978 and considered for allele assignment.

^cThe allele range for each reaction is indicated showing that amplicons with the same dye cannot overlap. Values obtained with A. baumannii isolates.