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. 2012 Sep 12;7(9):e45010. doi: 10.1371/journal.pone.0045010

Figure 6. The TSA-mediated positive effect on MEA testing is transient.

Figure 6

(A) Results of qRT-PCR analyses of cardiac gene expression in hESC-CMs showed that the effect of TSA was transient. Day 5 (d5): immediately after 2 days of TSA treatment; Day 10 (d10): 5 days of culture after TSA washout. Values relative to the DMSO-treated hESC-CMs (DMSO = 100%) are shown (n = 3). Values represent the mean ± SD for each set of measurements. (B) Sequential MEA analyses of all hESC-CM colonies were performed on Days 2, 6, and 9 in the presence of 200 nM E4031. Changes in the relative FPD values on Day 6 or Day 9 from Day 2 for each colony are shown in Figures 5 and 6B, respectively. Values represent the mean ± SD (n = 5 for DMSO, n = 6 for TSA). Significant differences between DMSO and TSA treatment detected on Day 6 were not detected on Day 9.