Figure 1. Impact of sample size on allele detection.

Percentage of samples in which all alleles at a real frequency ≥0.05 at each locus were detected at each sample size (of the 100 random replicates per size), for A) the ant dataset, B) the squirrel dataset, C) the albatross dataset and D) the kakī dataset. The vertical line shows the sample size at which all alleles at a real frequency ≥0.05 at all loci were detected in ≥95% of replicates.