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. 2012 Sep 12;7(9):e44943. doi: 10.1371/journal.pone.0044943

Figure 4. Adjuvant activity of IMX108/IMX313 in homologous prime-boost regimes.

Figure 4

Frequency of antigen-specific IFN-γ, TNFα and IL-2 positive CD4+ and CD8+ T cells in the spleen were measured by ICS fourteen days post-boost following two immunizations with 5×1010 vp AdHu5-PyMSP142±IMX108 i.d. (Ad-Ad42+IMX108 and Ad-Ad42-Nil respectively) (A), 5×107 pfu MVA-PyMSP142±IMX108 i.d. (M-M42+IMX108 and M-M42-Nil) (B) or 1×109 ifu AdHu5-PfAMA1±IMX313 i.m. (Ad-AdAMA1+IMX313 and Ad-AdAMA1-Nil) (C). Immunizations were given eight weeks apart. Box and whisker plots show median, IQR and range and pies show the proportion of cells positive for 1, 2 or all 3 cytokines. Antigen-specific total IgG responses were also measured in the serum fourteen days after boost by ELISA (D-F). Points represent individual mice and bars indicate median titer. The dashed line indicates the limit of detection. **P<0.01, *P<0.05 compared to the same vaccination regime with IMX108 or IMX313 by Mann Whitney test. Data show the results of single experiments.