Figure 5. Direct chromatin PCR for FOSB gene.

FOSB mRNA Expression after 10-E-09 In KMS-12-PE detected and analyzed on HT-12 Array™ (A). Cells were treated with 10-E-09 at 10 µM for indicated times and arrays were run in duplicates. Displayed are mean fluorescence units (Left panel) and standard deviations of duplicates (Right panel). Primer locations for DC-PCR are shown on a map of the FOSB 5′ region (B). An arrow indicates transcription start site for transcript NM_001114171.1. DC-PCR performed for FOSB on KMS-12-PE cells (C): untreated and treated with 10-E-09. Active chromatin of FOSB can be detected by DC-PCR 3 h after treatment with 10-E-09. Most of the 11 tested sequences allowed primer access whereas chromatin of untreated cells remained almost completely closed. Genomic DNA used as template demonstrated amplification of all FOSB sites. Displayed DC-PCR results are representative of three independent experiments.