Figure 7. Principles of DC-PCR drug screens.

Cartoon outlining the general procedure of DC-PCR and possible results that depend on the interrogated region and utilized cells (A). General outline of a CS-PCR screen for molecules that make the interrogated site more soluble (B). Only a fraction of cells (1–10 µl) is required to run the screen. Remaining cells can be used for confirmatory tests or secondary screens. If heat is used and confirmatory tests on hits are planned, an additional pipetting step before heating is required.