Table 1. Primers and probes.
Gen/Accession number | Upper primer | Lower primer | Amplicon (nt) | Probe number* |
Eag1/AF078741.1 | cct gga ggt gat cca aga tg | cca aac acg tct cct ttt cc | 60 | 49 |
Ki-67/X65550.1 | ggt gtg cag aaa atc caa aga | act gtc cct atg act tct ggt tg | 77 | 73 |
CYP24A1/NM_000782.3 | cat cat ggc cat caa aac aa | gca gct cga ctg gag tga c | 65 | 88 |
CYP3A4/NM_017460.3 | gat ggc tct cat ccc aga ctt | agt cca tgt gaa tgg gtt cc | 96 | 2 |
VDR/NM_000376.1 | gtg aga cct cac aga aga gca c | cat tgc ctc cat ccc tga | 72 | 68 |
GAPDH/AF261085.1 | agc cac atc gct gag aca c | gcc caa tac gac caa atc c | 66 | 60 |
From the universal probe library (Roche).