Table 3. Primers used in constructing the suicide plasmid and the mutant.

Name	Sequence
PMD fwd	ATAAGAATGCGGCCGCGCGGTAATACGGTTATCCAC
PMD rev	TCCGGAATTCGCGTCGACAATGGTTTCTTAGACGTCAGGT
Wholfwd	TCCGGAATTCGCGTCGACGTGAACACCGTGATCTTGTCC
Whol rev	ATAAGAATGCGGCCGCTCACAGTTCCGACGTTTTCAGT
Mid fwd	TCGGGGTACCGTTCCGATTTACCCAGGATCA
Mid rev	TTGGAAGATCTCTTCGATGACCATATCGAGACAG
Cat fwd	CTGAAGATCTTCATGTTTGACAGCT
Cat rev	TCGGGGTACCATTCATCCGCTTATTATCACTT
Clnfwd	TCCGCTCGAGGGTACCGTCTTAGAGCAAAGCGCCTGT
Cln rev	CTAGTCTAGAGCCGTAATCGGCGGAGTTTAT
SorAfwd	ATCCCATGGCCGGTCGTTAT
SorA rev	TGGTTGTGGATTTGTCGCAG
SorBfwd	ATCAGCTTGGAGGCAATGTG
SorB rev	ATGGTCTTGGGCTCTTGGTC
Big fwd	CGAGTCCCGCCCATTGTT
Big rev	TACAGGTAGAAGGCTTCACC