Figure 5. Transcriptional pattern of aphA during growth.

The bacterial cells were harvested at various OD₆₀₀ values during growth (according to the bacterial growth curves in Fig. 4) for total RNA isolation. An oligonucleotide primer was designed to be complementary to the RNA transcript of aphA. The primer extension products were analyzed with 8 M urea-6% acrylamide sequencing gel. Lanes C, T, A, and G represented Sanger sequencing reactions. The transcription start site of aphA was underlined in the DNA sequence.