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. 2012 Sep 12;7(9):e43609. doi: 10.1371/journal.pone.0043609

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Treg interference of dendritic cell paracrine secretion of IL-2 depends on CD25. C57BL/6 splenic DCs (left) and BMDCs (right) were cultured in IL-2 ELISPOT plates unstimulated or with CpG-B, with and without DO11.10 Treg cells, anti-CD25, or isotype control, and the number of IL-2 secreting DCs was measured as in materials and methods. Bars are the mean ± SE of IL-2 secreting DCs in triplicate samples of one of six representative experiments with splenic DCs (left) and one of three representative experiments with BMDCs (right). Statistical analysis by one-way repeated measures ANOVA and Tukey's test for all pairwise multiple comparison procedure.