Figure 6.

Biochemical properties of BC12.

(a) Constructs for characterization of BC12 biochemical features. Details of the constructs are given in the text.

(b) Western blotting of the GST affinity-purified GST- and His-tagged versions of the motor or coiled-coil domains using anti-His antibody.

(c) An SDS–PAGE gel stained with Coomassie Brilliant Blue to reveal co-sedimentation of the motor domain with (−) and without (+) microtubules (MT). P, pellet; S, supernatant.

(d) Absorbance of the reactions at 340 nm to reveal the ATPase activity of the purified motor domain in the presence of various amounts of microtubule (MT).

(e) Absorbance of the reactions at 340 nm to reveal the ATPase activity of the motor domain in the presence of 40 μg of microtubules.