Fig. 8.

Micrographs from time-lapse videos of cells in cortical astrocyte culture and fibroblasts cultured statically on porous PDMS membranes with defined pore features in perfusion culture modules for the cells to partly or completely seal the pores, then perfused continuously from left to right (as shown in Fig. 1C) with different flow rate (80, 160, 320 ml/h) or shear rate (1.1, 2.2, 4.4/s) for different periods of times and monitored using time-lapse video microscopy. Bar = 100 μm.