Figure 1.
Confirmation of cassava transformants with modified FEA1 gene. (A) PCR amplification of the FEA1 gene in transgenic cassava. WT, wild-type cassava; 3DF*, 3DF*B, 3DF*5, 3DF*6, 3DF*7, and 3DF*10 are transgenic cassava. (B) Analysis of FEA1 gene expression: (A) RT-PCR analysis of FEA1 gene expression in roots of transgenic and wild-type cassava plants. The cassava sbe1 gene was used as loading control. (C) RT-PCR analysis of FEA1 gene expression in leaves of transgenic and wild-type cassava plants. Loading control for (B) is cassava α-tubulin gene.