Figure 9.

Differential expression of metal regulated genes in FEA1 transgenic cassava. Relative expression (Q-RT-PCR) of metal regulated genes [(A); MeFro2, (B); MeIrt6, (C); MeYsl1, (D); MeFer1, (E); MeFer3] as indicated in wild-type and two independent FEA1 transgenic lines. Tissues (roots, stem, and leaves) were collected at 1.5-month-old in vitro stage plants. Expression of the metal regulated genes was normalized to alpha tubulin. Wild-type root expression levels were adjusted to a value of 1 and all other expression values were normalized relative to this tissue. For each sample, reactions were set up in quadruplicates using two biological replicates. Error bars represent SE. The asterisk (*) indicates statistically significant differences between wild-type and transgenics, determined by Student’s t-test, with P < 0.05.