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. 2012 Sep 13;3:171. doi: 10.3389/fpls.2012.00171

Table A1.

Forward primers for the codon-optimization of ChlamydomonasFEA1 gene for expression in cassava.

Orientation FEA1 Forward primers
F1 GCACAGTTAACCCCGGGATGTCTGTCGGATTTCTGGTCCTC
F2 GCTCTGGGCGCTCTTGTCGTGGCTACTGCTCAGCCAACTACT
F3 ACTGGCACTAGATTCGAGGGTTTCTCTTACGCTGGCAATGTC
F4 ATTGGCTATGTGAACATGACTATGGACTACTGCGACATCAAG
F5 GCCGCCATGGCTGCTGGCAACTTCACCGAGGCTCTGTCCATC
F6 TACTCTACTGGCAAGAACTCTTTCTCTGGCCTGGCTAGAAGA
F7 ACCTTCTTCAGATTCGCCTCTTACATCACCGCCAACGGCTCC
F8 GTGGAGCCACTGCACGACTCCATCCTGGCCGGCAAGGACACT
F9 TCCTCCCTGGACGCCGCCATCAGGGCTGCCCTGGCCGACGGC
F10 AAGGCCACCCTGGCCGCCGGTCTGATCCAGGTGGGCACTCTC
F11 AAGTACCACCTGCACGAGGTGGATGAGGCCTACAACAAGATC
F12 AAGACCTACCTGGCTGACGGCACCGGCAACCTCACCAACCTG
F13 GTTTCTGACGCCTCTGGCGCACCACACAACGTGGATGAGGCC
F14 TGGGCTCTGTGGGCCGGCGGCGCCGCCAACAACTGCGGCACC
F15 CTGTCCGGCTGGGCCTCCTCCCTGGGCGCCGCCATGGGCACC
F16 ACCTTCCTCGGCAAGAGCTATGTCAACACCGCCATGATCAAC
F17 ACCGTCAATGAGATGCTGGCCGCCGCCAGACTGTCTACCCTG
F18 AACATCCAAGCCTACGACGCCGCTAGAACTAACGAGGTCAGA
F19 CTGCTGACCCTGCTGGGCCTGCAGGGCGTGTCCGTGGCCGCT
F20 TACACCGCTGACGCCGCCGCCGCCTGCAAGAGACCAGCCGCC
F21 GAGGTGGAGGATGCCAAGACCATGATCGCCGTGCACTGGGCT
F22 TACCTGGAGCCTATGCTCAAGCTGAGAAACTTCAAGGCCTCC
F23 GCCGTGACCGAGCTGCACCACCAGCTCACCGCCTCCAAGCTG
F24 AGCTACAAGAAGGTGGCCGCCGCTGTGAAGGGCGTGCTGTCT
F25 GCTATGGGCAGAAGATCCAGCGAGCTGGGTGCCCCACAGTCC
F26 GCCATCATTGCCGCCAACTGGAAGTGCAGCTCCAAGACCCTG
F27 AGAAGCATTGCTTAATTGGGAACATGATGGCATTGTGCTGGA
F28 AGATTGGGACTTGACACTGACTAGTTAGATAGGGCAACTCAG
F29 GCAATAGGAATCGTTAGGACCTTTAGGGAAGCTGTCATGAGC
F30 ACTGTTTGCGTGTACATATCAGTTGTGGATGGAGAAGGTGGG
F31 TAATGCCACGTTGCCGCCTTCCAGTGACTGCAAAGAAGCTAT
F32 GCTTGATTGCTTTGAGGAAGGACTCAATGCTCATCAATGAGG
F33 GTCATGATGAAATTAAATGGCCAATGTGGGGGTAAGGTTCTT
F34 TCTATGACCCCAGGTTGAAGGGTTGTGTTTGTTTGCGGCACT
F35 TGAGGTCAGTGGTAAAGACACCCAGTGCCTGCCGCTTCAGCC
F36 TGTGATCAGCTCATATGCACAAAGGCTGGAGGAGAGGAGCTG