Table A2.

Reverse primers for codon-optimization of Chlamydomonas FEA1 gene for expression in cassava.

Orientation	FEA1 reverse primer 5′–3′
R1	CATGGAGAGCTCACAGTATTACATTACAGCTCCTCTCCTCCA
R2	GCCTTTGTGCATATGAGCTGATCACAGGCTGAAGCGGCAGGC
R3	ACTGGGTGTCTTTACCACTGACCTCAAGTGCCGCAAACAAAC
R4	ACAACCCTTCAACCTGGGGTCATAGAAAGAACCTTACCCCCA
R5	CATTGGCCATTTAATTTCATCATGACCCTCATTGATGAGCATT
R6	GAGTCCTTCCTCAAAGCAATCAAGCATAGCTTCTTTGCAGTC
R7	ACTGGAAGGCGGCAACGTGGCATTACCCACCTTCTCCATCCA
R8	CAACTGATATGTACACGCAAACAGTGCTCATGACAGCTTCCC
R9	TAAAGGTCCTAACGATTCCTATTGCCTGAGTTGCCCTATCTA
R10	ACTAGTCAGTGTCAAGTCCCAATCTTCCAGCACAATGCCATC
R11	ATGTTCCCAATTAAGCAATGCTTCTCAGGGTCTTGGAGCTGC
R12	ACTTCCAGTTGGCGGCAATGATGGCGGACTGTGGGGCACCCA
R13	GCTCGCTGGATCTTCTGCCCATAGCAGACAGCACGCCCTTCA
R14	CAGCGGCGGCCACCTTCTTGTAGCTCAGCTTGGAGGCGGTGA
R15	GCTGGTGGTGCAGCTCGGTCACGGCGGAGGCCTTGAAGTTTC
R16	TCAGCTTGAGCATAGGCTCCAGGTAAGCCCAGTGCACGGCGA
R17	TCATGGTCTTGGCATCCTCCACCTCGGCGGCTGGTCTCTTGC
R18	AGGCGGCGGCGGCGTCAGCGGTGTAAGCGGCCACGGACACGC
R19	CCTGCAGGCCCAGCAGGGTCAGCAGTCTGACCTCGTTAGTTC
R20	TAGCGGCGTCGTAGGCTTGGATGTTCAGGGTAGACAGTCTGG
R21	CGGCGGCCAGCATCTCATTGACGGTGTTGATCATGGCGGTGT
R22	TGACATAGCTCTTGCCGAGGAAGGTGGTGCCCATGGCGGCGC
R23	CCAGGGAGGAGGCCCAGCCGGACAGGGTGCCGCAGTTGTTGG
R24	CGGCGCCGCCGGCCCACAGAGCCCAGGCCTCATCCACGTTGT
R25	GTGGTGCGCCAGAGGCGTCAGAAACCAGGTTGGTGAGGTTGC
R26	CGGTGCCGTCAGCCAGGTAGGTCTTGATCTTGTTGTAGGCCT
R27	CATCCACCTCGTGCAGGTGGTACTTGAGAGTGCCCACCTGGA
R28	TCAGACCGGCGGCCAGGGTGGCCTTGCCGTCGGCCAGGGCAG
R29	CCCTGATGGCGGCGTCCAGGGAGGAAGTGTCCTTGCCGGCCA
R30	GGATGGAGTCGTGCAGTGGCTCCACGGAGCCGTTGGCGGTGA
R31	TGTAAGAGGCGAATCTGAAGAAGGTTCTTCTAGCCAGGCCAG
R32	AGAAAGAGTTCTTGCCAGTAGAGTAGATGGACAGAGCCTCGG
R33	TGAAGTTGCCAGCAGCCATGGCGGCCTTGATGTCGCAGTAGT
R34	CCATAGTCATGTTCACATAGCCAATGACATTGCCAGCGTAAG
R35	AGAAACCCTCGAATCTAGTGCCAGTAGTAGTTGGCTGAGCAG
R36	TAGCCACGACAAGAGCGCCCAGAGCGAGGACCAGAAATCCGA