Figure 1.
Analysis of the influence of LMWH on cell invasion. Boyden chambers (8 μm) were coated with collagen-IV, and cells (105) were placed in the upper chambers and made up to 250 μl with media containing a range of LMWH concentrations (0–2,000 μg/ml). Media (250 μl) containing 5 μg/ml of bFGF were placed in the lower chambers and incubated at 37°C for 24 h. The number of cells was then analysed [BxPC-3 cells shown in (A) by staining the cells with crystal violet and counting the cells on the bottom of the membrane]. The crystal violet was then released with 1% (w/v) SDS and the absorption of the samples was measured at 595 nm and quantified against a standard curve at 595 nm (B). (A) Micrographs are representative of three experiments; (B) the absorption data from which they were averaged are presented (*p<0.05 vs. untreated sample).