. 2011 Jan 20;2(2):363–367. doi: 10.3892/etm.2011.211

Table I.

Analysis of the influence of LMWH on TF antigen expression and activity.

A, TF antigen in ng per 10⁶ cells

LMWH conc. (μg/ml)	BxPC-3	LoVo	MDA-MB-231	SKOV-3	A375
0	112.3±15.5	116.4±14.6	135.0±7.6	94.4±8.9	100.8±4.4
20	81.5±6.0^a	96.6±14.6	75.1±8.9^a	83.0±6.6	85.5±2.3
200	81.0±8.4^a	85.0±17.4^a	29.1±3.9^a	12.6±8.2^a	82.7±3.0
2000	80.2±3.8^a	71.5±12.5^a	27.2±3.6^a	12.6±3.3^a	58±6.8^a

B, TF activity in equivalent units of TF per 10⁶ cells

LMWH conc. (μg/ml)	BxPC-3	LoVo	MDA-MB-231	SKOV-3	A375
0	250.6±10	432±35	250±16	207±11	±221
20	213.8±12^a	385±30	180±25^a	205±9	±209
200	195.7±17^a	258±13^a	160±3^a	120±8^a	±182
2000	179.8±18^a	235±28^a	150±7^a	100±1^a	±143^a

C, Correlation between TF antigen and TF activity

	BxPC-3	LoVo	MDA-MB-231	SKOV-3	A375
TF activity vs. TF antigen	0.896	0.949^a	0.977^a	0.986^a	0.962^a

Sets of cells (10⁶) were incubated with a range of LMWH concentrations (0–2,000 μg/ml) for 24 h.

(A) The cells were lysed and analysed by ELISA and TF concentrations were determined from a standard curve prepared alongside. Data represent the average of four experiments measured in duplicates (^ap<0.05 vs. untreated sample).

(B) The cells were resuspended in PBS (2×10⁵ cells/20 μl) and TF activity was measured using a chromogenic assay. The absorption values were then converted into equivalent concentrations from a standard curve. Data represent the average of four experiments measured in duplicates (^ap<0.05 vs. untreated sample).