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Western blot analysis of major cell cycle-regulating proteins. Caco-2 (A), SW-480 (B), CX-1 (C) and WIDR (D) cell lines were treated with increasing concentrations of valproate (VPA) over 5 days (0.25, 0.5, 1.0 and 2.0 mM) and subsequently analyzed. Representative results of three independent experiments are shown. β-actin served as an internal control.
