Figure 6.

(A) Treatment of male SCID mice with subcutaneous SW-480 xenografts with increasing doses of valproate (VPA) (100, 200 and 300 mg/kg bw) resulted in a significant reduction in tumor volume beginning 17 days after treatment until 24 days, compared to the control (solvent treatment). A maximum and significant growth reduction was noted with 200 mg/kg VPA. (B) Tissue samples were analyzed for H3 and H4 expression and their corresponding acetylation, aH3 and aH4. (C) Histone acetylation increased with VPA treatment with a maximum response at 200 mg/kg. Cdk1, 2 and 4, cyclin D1 and E as well as tumor suppressors p19, p21 and p27 expression under the influence of 100, 200 and 300 mg/kg VPA. Results from one representative experiment out of three are shown.