Figure 2.

BAAT1 interactions with molecules related to DNA damage response. (A) BAAT1 structural features. Two SNP sites (G20R and R644Q), one putative phosphorylation site (S742) and two HEAT repeat domains (H) are shown. (B) Map of the regions of interaction. To determine the binding region of BAAT1, 293T cells were transfected with the pEBG2 vector (V) or plasmids containing GST-fusion BAAT1 fragments. After 48 h, cells were treated with neocarzinostatin (NCS) or without. Total lysates were isolated and subjected to immunoprecipitation with GSH-sepharose beads as described previously (7). After immunoprecipitation, samples were immunoblotted with anti-DNA-PKcs, and protein levels were confirmed with anti-GST antibodies.