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Down-regulation of BAAT via siRNA. MCF10A normal human mammary epithelial cells were transfected with siRNA oligomer for BAAT1 knockdown as previously described (7). After 72 h, cells were treated with NCS for the indicated times. Total lysates were prepared and subjected to Western blotting. The level of total protein and phosphorylation by NCS were detected with the indicated antibodies. Actin protein was used as a loading control for immunoblotting.
