Figure 4.

The effect of the acute administration of vitamin B6 on aP2 mRNA expression in mouse adipose tissues. (A) Mice were fed a vitamin B6-free diet for 2 weeks and then received an oral administration of pyridoxine hydrochloride (200 or 500 mg/kg body weight) or physiological saline as control. After a 6-h administration of pyridoxine hydrochloride, 10 μg of total RNA from epididymal fat pads was isolated and subjected to Northern blot analysis. (B) The radioactivity in each band was quantified using an image analyzer and normalized to that of 18S. Each value relative to the mRNA level of the control mice represents the mean ± SE. (n=4). ^**P<0.01 vs. control.