Table I.
Information regarding the PCR primers.
| Primer | NCBI ID | Primer sequence | Product size (bp) | Melting temperature (°C) |
|---|---|---|---|---|
| β-catenin | NM_001098209.1 | F: CCCACTAATGTCCAGCGTTT | 382 | 54.0 |
| R: AACCAAGCATTTTCACCAGG | ||||
| Oct 3/4 | NM_002701.4 | F: TTCAGCCAAACGACCATC | 484 | 57.4 |
| R: GGAAAGGGACCGAGGAGTA | ||||
| SOX2 | NM_003106.2 | F: ACACCAATCCCATCCACACT | 223 | 61.0 |
| R: CAAACTTCCTGCAAAGCTCC | ||||
| MRP1 | NM_019898.2 | F: CTGAGTTCCTGCGTACCTAT | 212 | 56.2 |
| R: TTCTGCGGTGCTGTTGT | ||||
| BCRP | NM_004827.2 | F: GCCATTCTCCCAGTCA | 494 | 49.7 |
| R: GGGCGTCTATACACCAT | ||||
| Survivin | NM_001012271.1 | F: CTTGCCAGAGCCACGAA | 632 | 55.4 |
| R: GGAACCTCACCCATAGCC | ||||
| β-actin | NM_001101.3 | F: GCCCTGAGGCACTCTTC | 330 | 54.9 |
| R: GGCCGGACTCGTCATAC |
F, forward; R, reverse.