Table A1.
Composition of the cultivation media, adapted from de Kreuk et al. (2005) and Ebrahimi et al. (2010).
| Compound | CAS no. | Molecular formula | Molecular weight (g mol−1) | Amount per 20 L of medium1 | Concentration in medium (mmol L−1) |
|---|---|---|---|---|---|
| Carbon source medium2 | |||||
| Sodium acetate | 127-09-3 | C2H3O2Na ·3H2O | 136.09 | 170.11/212.64 g | 362.5/78.1 |
| Magnesium sulfate | 7487-88-9 | MgSO4·7H2O | 246.51 | 17.75 g | 3.6 |
| Potassium chloride | 7447-40-7 | KCl | 74.55 | 7.16 g | 4.8 |
| Nitrogen and phosphorus sources medium2 | |||||
| Ammonium chloride | 12125-02-9 | NH4Cl | 53.49 | 37.87 g | 35.4 |
| Dipotassium hydrogen phosphate | 7758-11-4 | K2HPO4 | 174.18 | 14.62 g | 4.2 |
| Potassium dihydrogen phosphate | 7778-77-0 | KH2PO4 | 136.09 | 5.72 g | 2.1 |
| Trace element solution | – | – | – | 100 mL | – |
| Compound | CAS no. | Molecular formula | Molecular weight (g mol−1) | Amount per 5 L of stock solution1 (g) | Concentration in stock solution (mmol L−1) |
| Trace element solution | |||||
| Disodium ethylenediaminetetraacetate | 139-33-3 | C10H14N2O8Na2·2H2O | 372.25 | 637.0 | 342.2 |
| Zinc sulfate | 7733-02-0 | ZnSO4·7H2O | 287.59 | 22.0 | 15.3 |
| Calcium chloride | 10043-52-4 | CaCl2·2H2O | 147.02 | 81.8 | 111.3 |
| Manganese chloride | 7773-01-5 | MnCl2·4H2O | 197.92 | 50.6 | 51.1 |
| Iron(II) sulfate | 7720-78-7 | FeSO4·7H2O | 278.05 | 49.9 | 35.9 |
| Ammonium heptamolybdate | 12027-67-7 | (NH4)6Mo7O24·4H2O | 1’235.88 | 16.4 | 2.7 |
| Copper(II) sulfate | 7758-98-7 | CuSO4·5H2O | 249.71 | 15.7 | 12.6 |
| Cobalt(II) chloride | 7646-79-9 | CoCl2·6H2O | 237.96 | 16.1 | 13.5 |
1The cultivation media and the trace element solution were prepared in 20 and 5 L of demineralized water.
2During the feeding phase of each SBR cycle, the synthetic influent wastewater was composed of 10% (v/v) of carbon source medium, of 10% (v/v) of nitrogen and phosphorus source medium, and of 80% (v/v) of tap water.
3The reactors R1–R5 and R6 were operated with 400 and 500 mgCOD L−1 of acetate in the influent wastewater, respectively. The chemical oxygen demand (COD) conversion factor of acetate is 64 gCOD molAc−1.