Table A2.

Phylogenetic affiliation of predominant clones isolated from biomass samples of reactors R1–R5.

T-RF1 (bp)	Reactor2	Affiliation3		Accession number3	Closest relative and original microbiota4	Sequence identity5 (%)
		Phylum → Family	Genus
71	R3	O: Rhodocyclales	G: Zoogloea	AJ505853	Zoogloea resiniphila strain PIV-3C2w from denitrifying bacterial community	99
185	R4	C: Gammaproteobacteria		AM180057	Uncultured bacterium clone A1-41 from a N- and P-removing system	97
		O: Rhizobiales	G: Ochrobactrum	CP000759	Ochrobactrum anthropi ATCC 49188	100
193	R4, R5	O: Rhodocyclales	G: Dechloromonas	AF314420	Uncultured bacterium PHOS-HE23 from aerobic EBPR system	99
195	R1, R3	O: Rhodocyclales	G: Zoogloea	DQ413172	Zoogloea sp. EMB 357 from anaerobic-aerobic SBR	100
	R4, R5	O: Rhodocyclales	G: Zoogloea	AF527582	Uncultured bacterium clone LPB19 from EBPR system	99
		O: Enterobacteriales	G: Escherichia/Shigella	AY838362	Bacterium HPC775 from effluent treatment plant of chemical and dye industry	98
208	R1, R3	O: Burkholderiales	G: Sphaerotilus	AB087568	Sphaerotilus sp. L19 involved in filamentous sludge bulking process	100
		O: Burkholderiales	G: Leptothrix	FM175128	Uncultured Leptothrix sp. from tufa core sample	98
	R4, R5	O: Burkholderiales		AB087576	Leptothrix sp. L18 involved in filamentous sludge bulking process	98
213	R5	O: Burkholderiales	F: Comamonadaceae	AY491594	Uncultured bacterium clone oc53 from oligotrophic microbial fuel cells	99
214	R4, R5	O: Rhodocyclales	F: Rhodocyclales	EF565147	Uncultured bacterium clone UWMH_4 in Accumulibacter enrichment	99
215	R3	O: Rhodocyclales	G: Dechloromonas	EF632559	Dechloromonas sp. A34 from phosphate mining overburden	98
	R4, R5	O: Rhodocyclales	G: Dechloromonas	AY823964	Uncultured Betaproteobacterium clone DFAW-071 from acetate-fed denitrifying community	100
216	R5	O: Nitrosomonadales	G: Nitrosomonas	AJ621027	Ammonium-oxidizing Nitrosomonas sp. Is32	97
217	R3	O: Rhodocyclales	G: Zoogloea	DQ413157	Zoogloea sp. EMB 108 from anaerobic-aerobic SBR	98
	R1	O: Rhodocyclales	G: Thauera	AM084110	Thauera sp. R-28312 from denitrifying community	95
224	R4, R5	O: Sphingomonadales	G: Novosphingobium	AJ746094	Novosphingobium sp. MG37 from haemodialysis water and fluid	100
		O: Rhodobacterales	F: Hyphomonadaceae	AF502221	Uncultured bacterium clone HP1B26 from EBPR sludge	99
239	R4, R5	C: Gammaproteobacteria		AY651306	Uncultured bacterium clone Cont82 from EBPR ecosystem	99
250	R3	O: Pseudomonadales	G: Acinetobacter	GQ383923	Acinetobacter sp. XJ127 from sewage water	99
	R4, R5	O: Pseudomonadales	G: Acinetobacter	DQ205305	Kartchner Caverns bacterium HI-O4	100
		O: Flavobacteriales	G: Chryseobacterium	AF502204	Uncultured bacterium clone HP1B06 from EBPR sludge	99
289	R4, R5	O: Sphingomonadales	F: Sphingomonadaceae	U37341	Sphingomonas paucimobilis isolate EPA 505 from PAH-degrading soil consortia	100

The initial cloning-sequencing database of Ebrahimi et al. (2010) was complemented in the present study.

¹Size of target terminal-restriction fragments (T-RF) obtained with HaeIII digestion and forming operational taxonomic units (OTU).

²Reactor biomass system from which the clones were isolated. A number of 1–7 clones were sequenced per T-RF.

³Closest bacterial affiliations and GenBank accession numbers obtain after mapping in the Ribosomal Database Project (RDP; Cole et al., 2009).

Legend: P, phylum; C, class; O, order; F, family; G, genus.

⁴Description of closest relatives and original microbiota from which the reference clones were isolated obtained from GenBank (Benson et al., 2011).

⁵Sequence identity scores obtained after mapping in RDP.