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mTORC1 inhibition after treatment with RAD001 or raptor siRNA has differential effects on the phosphorylation of AKT substrates. Cells were either treated with 20 nmol/L RAD001 for 48 h (A) or transfected with raptor (B) or rictor (C) siRNA for 72 h. Total protein extracts were analyzed by immunoblot for phosphorylation of the AKT substrates—GSK3β, FoxO1/3a, and TSC2.
