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AG1024 increases the apoptotic activity of gefitinib. After treatment with the different inhibitors for 24 h, cells were harvested and stained with 5 μl Annexin V-FITC and 5 μl propidium iodide (PI). Cells were then analyzed by flow cytometry. (A) Untreated group as the control; (B) AG1024 (5 μmol/l, 24 h) treatment group; (C) gefitinib (2.5 μmol/l, 24 h) treatment group; (D) gefitinib (2.5 μmol/l) combined with AG1024 (5 μmol/l, 24 h) treatment group.
