Table I.
Primer and probe sequences for quantitative RT-PCR.
| Gene | Gene symbol (HUGO) | GenBank no. | Forward primer (5′-3′) | Reverse primer (3′-5′) | TaqMan probe (5′-3′) |
|---|---|---|---|---|---|
| TS | TYMS | NM_001071.2 | GCCTCGGTGTGCCTTTCA | CCCGTGATGTGCGCAAT | TCGCCAGCTACGCCCTGCTCA |
| DPD | DPYD | NM_000110.3 | AGGACGCAAGGAGGGTTTG | GTCCGCCGAGTCCTTACTGA | CAGTGCCTACAGTCTCGAGTCTGCCAGTG |
| hENT1 | SLC29A1 | NM_004955.2 | CCAAGTTGGACCTCATTAGCA | TGGGCTGAGAGTTGGAGACT | TGCCTGCTCTTGGCTCCTCTCC |
| RRM1 | RRM1 | NM_111033.3 | ACTAAGCACCCTGACTATGCTATCC | CTTCCATCACATCACTGAACACTTT | CAGCCAGGATCGCTGTCTCTAACTTGCA |
| RRM2 | RRM2 | NM_001034.2 | ACCGCGAGGAGGATCT | TCAGCAGCGGCTCATC | TTTCGGCTCCGTGGGCTCCT |
| DHFR | DHFR | NM_000791.3 | GTCCTCCCGCTGCTGTCA | GCCGATGCCCATGTTCTG | TTCGCTAAACTGCATCGTCGCTGTGTC |
| FPGS | FPGS | M98045 | GGCTGGAGGAGACCAAGGAT | CATGAGTGTCAGGAAGCGGA | CAGCTGTGTCTCCATGCCCCCCTAC |
| GGH | GGH | NM_003878.2 | GTGGCAATGCCGCTGAA | CAACTCAGTAGGAAAATTCTGGAACA | TTCACTGGAGGTCAATTGCACAGCAGA |
| EGFR | EGFR | NM_005228.3 | TGCGTCTCTTGCCGGAAT | GGCTCACCCTCCAGAAGGTT | ACGCATTCCCTGCCTCGGCTG |
| VEGF | VEGFA | NM_003376.4 | AGTGGTCCCAGGCTGCAC | TCCATGAACTTCACCACTTCGT | TGATTCTGCCCTCCTCCTTCTGCCAT |
| β-actin | ACTB | NM_001101.3 | GAGCGCGGCTACAGCTT | TCCTTAATGTCACGCACGATTT | ACCACCACGGCCGAGCGG |