. 2011 Sep 1;2(6):1199–1204. doi: 10.3892/etm.2011.346

Table I.

Extracellular proteins identified by MALDI-TOF mass-spectrometry in the exponential growth phase of S. aureus strain 6.

N	Accession no.	Gene symbol	Ordered locus name	Protein identification	MW (Da)	pI	Protein score	Coincident peptides	The most similar strains	Localization according to PSORTb
N	Accession no.	Gene symbol	Ordered locus name	Protein identification	MW (Da)	pI	Protein score	Coincident peptides	The most similar strains	CP	CM	CW	EC	Total
1	Q2YWF3	fba	SAB2479	Fructose bisphosphate aldolase class 1	32907	4.69	788	25	RF122, MRSA252	2.50	2.50	2.50	2.50	U
2	B89855	gcvH	SA0760	Glycine cleavage system H protein	14072	3.70	140	2	N315, RF122, NCTC 8325, MW2	2.50	2.50	2.50	2.50	U
3	A89896	fabD	SA1073	Malonyl CoA-acyl carrier protein transacylase	33628	4.59	97	3	N315, RF122, MRSA252, NCTC 8325, MRSA476	9.67	0.01	0.15	0.17	CP
4	H89832	SA0587	SA0587	Lipoprotein, Streptococcal adhesin PsaA homologue	35049	9.16	120	2	N315	0.00	9.68	0.17	0.16	CM^a
5	F89850	pgk	SA0728	Phosphoglycerate kinase	42575	4.96	373	14	N315, RF122	10.00	0.00	0.00	0.00	CP
6	D89795	SA0295	SA0295	Similar to outer membrane protein precursor	33331	10.08	353	20	N315, MRSA252	0.00	3.33	3.33	3.33	U^a
7	H89862	glpQ	SA0820	Putative glycerophosphoryl diester phosphodiesterase	35289	9.14	87	2	N315, MRSA252	0.00	0.15	4.55	5.15	U^a
8	D89825	SAS0528	SAS0528	Putative hexulose-6-phosphate synthase	22422	4.32	244	3	N315, RF122, MW2, USA300	2.50	2.50	2.50	2.50	U
9	H89935	sodA	SA1382	Superoxide dismutase	22697	4.86	387	17	N315	0.01	0.09	0.18	9.72	EC
10	F89963	SA1599	SA1599	Similar to transaldolase	25742	4.51	260	7	N315, RF122, JH1	2.50	2.50	2.50	2.50	U
11	G89850	tpiA	SA0729	Triosephosphate isomerase	27245	4.51	539	19	N315, RF122, NCTC 8325	9.67	0.01	0.15	0.17	CP

The Mascot search results displayed above [accession no., molecular weight (MW), protein score, coincident peptides and the most similar strains) are from the best protein match to published S. aureus genomes. Gene designations and ordered locus names are according to UniProt (http://www.uniprot.org) for S. aureusN315. pI values were determined using Isoelectric Point Calculator (http://isoelectric.ovh.org) and EMBOSS (http://emboss.sourceforge.net) pK values. Localization was predicted using PSORTb 3.0 (http://psort.org/psortb/index.html). CP, cytoplasmic; CM, cytoplasmic membrane; CW, cell wall-associated; EC, extracellular. Protein localization was based upon which category scored the highest value as determined by PSORTb. If localization could not be determined by PSORTb, the protein was listed as unknown (U).

Presence of the consensus amino acid sequence for N-terminal signal peptide.